d d solubilizer (TaKaRa)
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TaKaRa
d d solubilizer
D D Solubilizer, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 90 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/d+d+solubilizer/D%2FD+Solubilizer/pm41912567-301-2-5
Average 96 stars, based on 90 article reviews
D D Solubilizer, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 90 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/d+d+solubilizer/D%2FD+Solubilizer/pm41912567-301-2-5
Average 96 stars, based on 90 article reviews
d d solubilizer - by Bioz Stars,
2026-09
96/100 stars
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Inverted Microscopy:Article Title: Mad1 facilitates α5 integrin trafficking from the Golgi to promote abscission during cytokinesis. Article Snippet: .. AR TI CL E IN P RE SS For 4xDmrD-Halo-GFP-α5TMCT live trafficking experiments, Mannosidase II-mApple and 4xDmrD-Halo-GFP-α5TMCT labelled cells were treated with Construct:Article Title: The role of disulfide bonds in the GluN1 subunit in the early trafficking and functional properties of GluN1/GluN2 and GluN1/GluN3 NMDA receptors Article Snippet: Cells were washed with ice-cold PBS and fixed in a solution containing 4% 221 paraformaldehyde (PFA; w/v; Merck) with 4% sucrose (w/v; Merck) in PBS for 15 min. 222 Subsequently, the cells were permeabilized with 0.25% Triton X-100 (v/v; Merck) in PBS for 5 min 223 and incubated with primary antibody (mouse anti-GFP, 1:1000; UC Davis/NIH NeuroMab Facility; 224 Cat# N86/8; RRID: AB_2313651) and then secondary antibody (goat anti-mouse IgG conjugated 225 with Alexa Fluor 488, 1:1000; Thermo Fisher Scientific; Cat# A-11001; RRID: AB_2534069) to 226 label intracellular antigens (Skrenkova et al., 2020; Kolcheva et al., 2023). .. In experiments with 227 ARIAD-GluN1-1a and ARIAD-hGluN1-1a constructs, cells were first incubated with ARIAD ligand 228 at a concentration of 1 μM (AL; Article Title: The role of disulfide bonds in the GluN1 subunit in the early trafficking and functional properties of GluN1/GluN2 and GluN1/GluN3 NMDA receptors Article Snippet: Subsequently, the cells were permeabilized with 0.25% Triton X-100 (v/v; Merck) in PBS for 5 min and incubated with primary antibody (mouse anti-GFP, 1:1000; UC Davis/NIH NeuroMab Facility; Cat# N86/8; RRID: AB_2313651) and then secondary antibody (goat anti-mouse IgG conjugated with Alexa Fluor 488, 1:1000; Thermo Fisher Scientific; Cat# A-11001; RRID: AB_2534069) to label intracellular antigens ( ; ). .. In experiments with ARIAD-GluN1-1a and ARIAD-hGluN1-1a constructs, cells were first incubated with ARIAD ligand at a concentration of 1 μM (AL; Article Title: Mad1 facilitates α5 integrin trafficking from the Golgi to promote abscission during cytokinesis. Article Snippet: .. Addition of Article Title: The Role of Disulfide Bonds in the GluN1 Subunit in the Early Trafficking and Functional Properties of GluN1/GluN2 and GluN1/GluN3 NMDA Receptors Article Snippet: Subsequently, the cells were permeabilized with 0.25% Triton X-100 (v/v; Merck) in PBS for 5 min and incubated with primary antibody (mouse anti-GFP, 1:1,000; UC Davis/NIH NeuroMab Facility; catalog #N86/8; RRID: AB_2313651) and then secondary antibody (goat anti-mouse IgG conjugated with Alexa Fluor 488, 1:1,000; Thermo Fisher Scientific; catalog #A-11001; RRID: AB_2534069) to label intracellular antigens ( ; ). .. In experiments with ARIAD-GluN1-1a and ARIAD-hGluN1-1a constructs, cells were first incubated with ARIAD ligand at a concentration of 1 μM (AL; Incubation:Article Title: The role of disulfide bonds in the GluN1 subunit in the early trafficking and functional properties of GluN1/GluN2 and GluN1/GluN3 NMDA receptors Article Snippet: Cells were washed with ice-cold PBS and fixed in a solution containing 4% 221 paraformaldehyde (PFA; w/v; Merck) with 4% sucrose (w/v; Merck) in PBS for 15 min. 222 Subsequently, the cells were permeabilized with 0.25% Triton X-100 (v/v; Merck) in PBS for 5 min 223 and incubated with primary antibody (mouse anti-GFP, 1:1000; UC Davis/NIH NeuroMab Facility; 224 Cat# N86/8; RRID: AB_2313651) and then secondary antibody (goat anti-mouse IgG conjugated 225 with Alexa Fluor 488, 1:1000; Thermo Fisher Scientific; Cat# A-11001; RRID: AB_2534069) to 226 label intracellular antigens (Skrenkova et al., 2020; Kolcheva et al., 2023). .. In experiments with 227 ARIAD-GluN1-1a and ARIAD-hGluN1-1a constructs, cells were first incubated with ARIAD ligand 228 at a concentration of 1 μM (AL; Article Title: The role of disulfide bonds in the GluN1 subunit in the early trafficking and functional properties of GluN1/GluN2 and GluN1/GluN3 NMDA receptors Article Snippet: Subsequently, the cells were permeabilized with 0.25% Triton X-100 (v/v; Merck) in PBS for 5 min and incubated with primary antibody (mouse anti-GFP, 1:1000; UC Davis/NIH NeuroMab Facility; Cat# N86/8; RRID: AB_2313651) and then secondary antibody (goat anti-mouse IgG conjugated with Alexa Fluor 488, 1:1000; Thermo Fisher Scientific; Cat# A-11001; RRID: AB_2534069) to label intracellular antigens ( ; ). .. In experiments with ARIAD-GluN1-1a and ARIAD-hGluN1-1a constructs, cells were first incubated with ARIAD ligand at a concentration of 1 μM (AL; Article Title: The Role of Disulfide Bonds in the GluN1 Subunit in the Early Trafficking and Functional Properties of GluN1/GluN2 and GluN1/GluN3 NMDA Receptors Article Snippet: Subsequently, the cells were permeabilized with 0.25% Triton X-100 (v/v; Merck) in PBS for 5 min and incubated with primary antibody (mouse anti-GFP, 1:1,000; UC Davis/NIH NeuroMab Facility; catalog #N86/8; RRID: AB_2313651) and then secondary antibody (goat anti-mouse IgG conjugated with Alexa Fluor 488, 1:1,000; Thermo Fisher Scientific; catalog #A-11001; RRID: AB_2534069) to label intracellular antigens ( ; ). .. In experiments with ARIAD-GluN1-1a and ARIAD-hGluN1-1a constructs, cells were first incubated with ARIAD ligand at a concentration of 1 μM (AL; Concentration Assay:Article Title: The role of disulfide bonds in the GluN1 subunit in the early trafficking and functional properties of GluN1/GluN2 and GluN1/GluN3 NMDA receptors Article Snippet: Cells were washed with ice-cold PBS and fixed in a solution containing 4% 221 paraformaldehyde (PFA; w/v; Merck) with 4% sucrose (w/v; Merck) in PBS for 15 min. 222 Subsequently, the cells were permeabilized with 0.25% Triton X-100 (v/v; Merck) in PBS for 5 min 223 and incubated with primary antibody (mouse anti-GFP, 1:1000; UC Davis/NIH NeuroMab Facility; 224 Cat# N86/8; RRID: AB_2313651) and then secondary antibody (goat anti-mouse IgG conjugated 225 with Alexa Fluor 488, 1:1000; Thermo Fisher Scientific; Cat# A-11001; RRID: AB_2534069) to 226 label intracellular antigens (Skrenkova et al., 2020; Kolcheva et al., 2023). .. In experiments with 227 ARIAD-GluN1-1a and ARIAD-hGluN1-1a constructs, cells were first incubated with ARIAD ligand 228 at a concentration of 1 μM (AL; Article Title: The role of disulfide bonds in the GluN1 subunit in the early trafficking and functional properties of GluN1/GluN2 and GluN1/GluN3 NMDA receptors Article Snippet: Subsequently, the cells were permeabilized with 0.25% Triton X-100 (v/v; Merck) in PBS for 5 min and incubated with primary antibody (mouse anti-GFP, 1:1000; UC Davis/NIH NeuroMab Facility; Cat# N86/8; RRID: AB_2313651) and then secondary antibody (goat anti-mouse IgG conjugated with Alexa Fluor 488, 1:1000; Thermo Fisher Scientific; Cat# A-11001; RRID: AB_2534069) to label intracellular antigens ( ; ). .. In experiments with ARIAD-GluN1-1a and ARIAD-hGluN1-1a constructs, cells were first incubated with ARIAD ligand at a concentration of 1 μM (AL; Article Title: The Role of Disulfide Bonds in the GluN1 Subunit in the Early Trafficking and Functional Properties of GluN1/GluN2 and GluN1/GluN3 NMDA Receptors Article Snippet: Subsequently, the cells were permeabilized with 0.25% Triton X-100 (v/v; Merck) in PBS for 5 min and incubated with primary antibody (mouse anti-GFP, 1:1,000; UC Davis/NIH NeuroMab Facility; catalog #N86/8; RRID: AB_2313651) and then secondary antibody (goat anti-mouse IgG conjugated with Alexa Fluor 488, 1:1,000; Thermo Fisher Scientific; catalog #A-11001; RRID: AB_2534069) to label intracellular antigens ( ; ). .. In experiments with ARIAD-GluN1-1a and ARIAD-hGluN1-1a constructs, cells were first incubated with ARIAD ligand at a concentration of 1 μM (AL; Labeling:Article Title: The role of disulfide bonds in the GluN1 subunit in the early trafficking and functional properties of GluN1/GluN2 and GluN1/GluN3 NMDA receptors Article Snippet: Cells were washed with ice-cold PBS and fixed in a solution containing 4% 221 paraformaldehyde (PFA; w/v; Merck) with 4% sucrose (w/v; Merck) in PBS for 15 min. 222 Subsequently, the cells were permeabilized with 0.25% Triton X-100 (v/v; Merck) in PBS for 5 min 223 and incubated with primary antibody (mouse anti-GFP, 1:1000; UC Davis/NIH NeuroMab Facility; 224 Cat# N86/8; RRID: AB_2313651) and then secondary antibody (goat anti-mouse IgG conjugated 225 with Alexa Fluor 488, 1:1000; Thermo Fisher Scientific; Cat# A-11001; RRID: AB_2534069) to 226 label intracellular antigens (Skrenkova et al., 2020; Kolcheva et al., 2023). .. In experiments with 227 ARIAD-GluN1-1a and ARIAD-hGluN1-1a constructs, cells were first incubated with ARIAD ligand 228 at a concentration of 1 μM (AL; Article Title: The role of disulfide bonds in the GluN1 subunit in the early trafficking and functional properties of GluN1/GluN2 and GluN1/GluN3 NMDA receptors Article Snippet: Subsequently, the cells were permeabilized with 0.25% Triton X-100 (v/v; Merck) in PBS for 5 min and incubated with primary antibody (mouse anti-GFP, 1:1000; UC Davis/NIH NeuroMab Facility; Cat# N86/8; RRID: AB_2313651) and then secondary antibody (goat anti-mouse IgG conjugated with Alexa Fluor 488, 1:1000; Thermo Fisher Scientific; Cat# A-11001; RRID: AB_2534069) to label intracellular antigens ( ; ). .. In experiments with ARIAD-GluN1-1a and ARIAD-hGluN1-1a constructs, cells were first incubated with ARIAD ligand at a concentration of 1 μM (AL; Article Title: The Role of Disulfide Bonds in the GluN1 Subunit in the Early Trafficking and Functional Properties of GluN1/GluN2 and GluN1/GluN3 NMDA Receptors Article Snippet: Subsequently, the cells were permeabilized with 0.25% Triton X-100 (v/v; Merck) in PBS for 5 min and incubated with primary antibody (mouse anti-GFP, 1:1,000; UC Davis/NIH NeuroMab Facility; catalog #N86/8; RRID: AB_2313651) and then secondary antibody (goat anti-mouse IgG conjugated with Alexa Fluor 488, 1:1,000; Thermo Fisher Scientific; catalog #A-11001; RRID: AB_2534069) to label intracellular antigens ( ; ). .. In experiments with ARIAD-GluN1-1a and ARIAD-hGluN1-1a constructs, cells were first incubated with ARIAD ligand at a concentration of 1 μM (AL; Clinical Proteomics:Article Title: Mad1 facilitates α5 integrin trafficking from the Golgi to promote abscission during cytokinesis. Article Snippet: .. Addition of Membrane:Article Title: Mad1 facilitates α5 integrin trafficking from the Golgi to promote abscission during cytokinesis. Article Snippet: .. Addition of |